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GHK-Cu dosage in studies: doses, timing and reconstitution

The cited studies used very different amounts: topical skin care products in the one human trial (concentration not stated in the abstract), 0.2 and 2 mg/kg in a mouse muscle model, and 1 × 10⁻⁹ mol/L in cultured fibroblasts. Several animal abstracts do not state a dose at all. This is a report of study methods, not a guide.

What GHK-Cu dosage did the studies use? The answer depends on the model. The only human trial here used topical skin care products after laser resurfacing for 12 weeks, and its abstract does not give the GHK-Cu concentration. The mouse muscle study used 0.2 and 2 mg/kg without stating the route in the abstract. The fibroblast study used 1 × 10⁻⁹ mol/L in culture medium.

Amounts across these models do not convert into one another. A mg/kg figure in mice, a molar concentration in a culture dish and a cream applied to skin measure different things, and none of them is a human injection dose. The mouse asthma, silicosis, scald and rat wound abstracts do not state the amount used.

The site's reconstitution calculator does the arithmetic of powder mass and diluent volume for a vial. It does not suggest an amount for any use.

Doses used by each cited study

StudyModelDoseFrequencyDuration
Miller TR et al. 2006Human RCT, post-laser skin (n=13 completed)Topical skin care products; concentration not stated in the abstractNot stated in the abstract12 weeks
Deng M et al. 2023Mouse, smoke-induced muscle dysfunction0.2 and 2 mg/kg (route not stated in the abstract)Not stated in the abstractNot stated in the abstract
Wang X et al. 2017Mouse, scald burnGHK-Cu liposomes; amount not stated in the abstractNot stated in the abstractHealing time 14 days post injury
Li H et al. 2015Human skin in vitro (microneedle pre-treatment)Not stated in the abstractSingle application9 hours
Pollard JD et al. 2005Cell culture, human dermal fibroblasts1 × 10⁻⁹ mol/LNot stated in the abstractNot stated in the abstract

This table reports what each study used. It is not a recommendation or an instruction.

Vial maths

VialBAC waterConcentrationPer 0.01 mL (1 unit, U-100)
50 mg1 mL50 mg/mL500 mcg
50 mg2 mL25 mg/mL250 mcg
50 mg3 mL16.667 mg/mL166.7 mcg
100 mg1 mL100 mg/mL1000 mcg
100 mg2 mL50 mg/mL500 mcg
100 mg3 mL33.333 mg/mL333.3 mcg

Open the calculator (pre-set for GHK-Cu)